Thirty 10-year-old Banaty grapevines were used in this investigation during 2022 and 2023 growing seasons cultivated in a private vineyard in West Abu Qurqas center, El-Minia Governorate, Egypt. Tested the effects of spraying algae extracts at (0.25, 0.50 and 0.75 g/L) and chitosan at (0.05, 0.1 and 0.2%) as well as their interactions with total 10 treatments in a Completely Randomized Block design with three replicates per treatment. The findings indicated that all treatments significantly enhanced various aspects of grapevine growth and leaves pigments and nutrient content. Moreover, the noted beneficial benefits were typically commensurate with the escalating doses of these treatments. Nevertheless, the results indicated no substantial disparities between the medium and elevated quantities of these substances. The most significant advantages were noted with the application of chitosan as individual application. The treatment that included three sprays of algae extracts (0.50 g/L) plus chitosan (0.1%) was the most economically treatment in improving Banaty grapevines vegetative growth and leaves chemical content under Minia conditions.