https://doi.org/10.37229/fsa.fjb.2026.04.25

This study was conducted in a private mushroom farm in Nineveh Governorate during 2024 to evaluate the effects of two strains of white button mushroom (Agaricus bisporus) grown on two cultivation media (grain straw and maize residues) and three concentrations of micronutrients (0,1, and 2 g L⁻¹). A Completely Randomized Design (CRD) with three replicates was used to arrange the experiment. The data obtained were statistically analyzed, and the means of treatments were separated according to Duncan’s Multiple Range Test at a probability level of 0.05. level (Al-Rawi & Khalafallah, 2000). The results indicated that the Dutch strain significantly surpassed the Indian strain in mycelial growth rate and number of fruiting bodies, producing the highest number of fruits per experimental unit (83). However, yield traits showed no significant differences between strains, although the Dutch strain recorded the highest total yield (13.6 kg. m^⁻²). Regarding growing media, the grain straw substrate achieved superior performance, producing the highest total yield (14.6 kg m^⁻²). Micronutrient supplementation, particularly at 2 g L⁻¹, significantly enhanced yield, reaching 14.3 kg m⁻². In terms of qualitative characteristics, the Dutch strain exhibited higher nitrogen content in fruiting bodies (647mg 100 g⁻¹). Analysis of cultivation media showed that the grain straw medium resulted in the highest nitrogen content (643mg 100g⁻¹). Furthermore, the application of micronutrients at 2g. L⁻¹ significantly increased nitrogen content in fruiting bodies, reaching 700 mg 100 g⁻¹.