The present investigation focused on the response of fruit physico-chemical parameters of ‘Medjool’ date palm to pollen grains source. However, three pollinator’s cvs namely; Ghanami, Jarvis and Boyer were examined. Furthermore, increasing the frequencies of pollination from two to four times were studied also. The obtained data showed that the pollinator type significantly varied the fruit physical properties (i.e. fruit weight, seed weight, pulp weight, pulp: seed ratio, fruit length and fruit diameter of ‘Medjool’ date palm, during the two experimental seasons. In this concern, the palms pollinated with ‘Ghanami’ shows significantly superior rather than those pollinated with ‘Jarvis’ or ‘Boyer’ pollinators. Furthermore, pollinator type had a significant effect on fruit reducing and non-reducing sugars, total acidity, crude fiber and soluble tannins. Whereas, in this concern Ghanami pollinators present superiority compared to using Jarvis or Boyer cvs. as a pollinator. While, non-significant differences were observed in fruit pulp TSS% and total sugars %. Furthermore, increasing the frequencies of pollination significantly improved fruit physical and chemical properties. While, non-significant differences in most physical and chemical properties as a result of increasing the frequencies of pollination from three to four times.