The effect of salinity stress on ‘Flame Seedless’ and ‘Superior Seedless’ grapevines seedlings (Vitis vinifera L.) grown hydroponically under salinity stress conditions, as well as the effectiveness of inoculation with Arbuscular Mycorrhizae Fungi (AMF) in reducing the harmful effects of salinity stress were investigated during 2022 and 2023 seasons. The investigation was carried out under Laboratory conditions, whereas the temperature was fixed at   25 ± 3 and relative humidity at 80 ± 5, referenced nutrient solution adapted with grapevines needs was used. The obtained results confirmed that, all vegetative parameters, fresh and dry weight of shoot and root systems, eaves main pigments and mineral status of both cultivars were negatively affected by increasing the salinity level in the nutrient solution. However, inoculated the seedlings with AMF significantly reduced these harmful effects of both cultivars. During both experimental seasons, the response of Flame Seedless seedlings to AMF inoculations was more perspicuous rather than those of Superior Seedless seedlings, these findings were true in most studied traits.