This study was carried out during the 2023-2024 growing season in the lath house of the Department of Horticulture and Landscape Design / College of Agriculture and Forestry / University of Mosul to know the response of fig seedlings of Waziri and Black Diyala cultivars and the effect of adding urea chemical fertilizer and Ferttybyo bacterial biofertilizer on the availability of nutrients in the soil and its acidity. The study included three factors: bacterial biofertilizer (Ferttybyo), which was added at three levels: 0,5 and 10 ml L^-1, and urea chemical fertilizer, which was added at three levels: 0, 5 and 10 g seedling^-1. The Ferttybyo 5 ml biofertilizer treatment showed a significant superiority in Soil pH, available phosphorus and potassium concentration were significant in the soil, while the 10 ml sauce treatment showed significant differences in the available phosphorus and nitrogen concentration in the soil, while the urea fertilizer treatment, at both levels of 5 and 10 g seedling^-1, gave significant differences in the soil pH, available phosphorus and potassium in the soil. All bilateral interactions played a major role in the growth of fig seedlings for both varieties, especially the interaction coefficients between Ferttybya biofertilizer and urea, which had a major impact in giving the best results in all studied characteristics except for the soil acidity assessment characteristic. All triple interaction coefficients between varieties, biofertilizer and urea indicated significant differences in all studied characteristics